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streptavidin af488  (Thermo Fisher)


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    Structured Review

    Thermo Fisher streptavidin af488
    Streptavidin Af488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/streptavidin+af488/Triton+X-100/pm42207645-386-5-36
    Average 99 stars, based on 1 article reviews
    streptavidin af488 - by Bioz Stars, 2026-10
    99/100 stars

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    Staining:

    Article Title: Protective and pathogenic antibody responses from a primate Shigella outbreak inform vaccine design
    Article Snippet: .. Cells were then stained with the antigen of interest (OMVs labeled with Vybrant TM DiO or DiD solutions [Thermo Fisher], IpaD labeled with biotin [Avidity BirA500] and coupled to streptavidin-AF488 and -AF647 [Thermo Fisher], or IpaB labeled with NHS esters of AF488 and AF647 [Thermo Fisher]). .. Cells were then stained with the antigen of interest (OMVs labeled with Vybrant TM DiO or DiD solutions [Thermo Fisher], IpaD labeled with biotin [Avidity BirA500] and coupled to streptavidin-AF488 and -AF647 [Thermo Fisher], or IpaB labeled with NHS esters of AF488 and AF647 [Thermo Fisher]).

    Article Title: Genetically diverse influenza antibodies highlight the role of IG germline gene variation and inform population-comprehensive vaccine strategies.
    Article Snippet: Fluorochrome-conjugated anti-human mAbs detecting cell surface CD19, CD20, IgM, IgD and IgG (BD Biosciences, BioLegend) were used to stain PBMCs. .. Biotinylated H1pdm2009-like influenza A monomeric head, trimeric stem domain and full trimer probes 39 were conjugated to ExtrAvidin®-PE (Sigma-Aldrich), Streptavidin-APC (Thermo Fisher) or Streptavidin-AF488 (Thermo Fisher) fluorophores and incubated with the stained cells. .. HA-antigen specific single cell sorting was performed using a FACSAria Fusion cell sorter (BD Biosciences) and target B memory cell populations were gated as live CD19+CD20+IgM–IgD–IgG+HA-trimer/head/stem+.

    Article Title: An inflammatory T-cell-stromal axis contributes to hematopoietic stem/progenitor cell failure and clonal evolution in human myelodysplastic syndrome
    Article Snippet: For isolation of the non-hematopoietic fraction, 90% of the thawed cells (>200 × 10 6 ) were stained with biotinylated antibodies against CD45 (1:50, clone: HI30, BioLegend) and CD235a (1:50, clone: HIR2, BioLegend) followed by depletion using magnetic anti-biotin beads (20 μL per 10 7 cells; Miltenyi Biotec) in PBS supplemented with 2% FCS and iMag (BD Biosciences). .. After depletion, the remaining cells were stained for sorting in PBS containing 0.5% FCS at 4 °C with the following antibodies: streptavidin-AF488 (1:100, Invitrogen), CD45-BV510 (1:50, clone HI30, BioLegend), CD235a-PE-Cy7 (1:50, clone: HI264, BioLegend), CD71-AF700 (1:20, clone: MEM-75, Exbio), CD271-PE (1:50, clone: ME20.4, BioLegend), CD105-APC (1:50, clone: SN6, eBioscience), CD31-APC-Cy7 (1:20, clone: WM59, BioLegend), CD34-eFluor610 (1:50, clone: 4H11, eBioscience), and CD144-V450 (1:50, clone: 55-7H1, BD Biosciences). .. 7AAD (1:100, Beckman Coulter) was used for dead cell exclusion.

    Article Title: Genetically diverse influenza antibodies highlight the role of IG germline gene variation and inform population-comprehensive vaccine strategies
    Article Snippet: Fluorochrome-conjugated anti-human mAbs detecting cell surface CD19, CD20, IgM, IgD and IgG (BD Biosciences, BioLegend) were used to stain PBMCs. .. Biotinylated H1 pdm2009 -like influenza A monomeric head, trimeric stem domain and full trimer probes were conjugated to ExtrAvidin ® -PE (Sigma-Aldrich), Streptavidin-APC (Thermo Fisher) or Streptavidin-AF488 (Thermo Fisher) fluorophores and incubated with the stained cells. .. HA-antigen specific single cell sorting was performed using a FACSAria Fusion cell sorter (BD Biosciences) and target B memory cell populations were gated as live CD19 + CD20 + IgM − IgD − IgG + HA-trimer/head/stem + .

    Article Title: Generation of an Induced Pluripotent Stem Cell‐Derived Alveolar Type II In Vitro Model to Study Influenza A Virus Infection and Drug Treatments
    Article Snippet: Nine days after seeding, cells were washed once with PBS in apical and basal wells, fixed with paraformaldehyde (3.7%; Carl Roth, 7398.1) in both wells for 15 min at RT, and washed thrice with PBS before PBS was removed from apical and basal wells. .. Cells were washed thrice in PBS and stained with streptavidin‐AF488 (3.3 μg mL −1 for SNA or 10 μg mL −1 for MAH; Invitrogen, S‐11223), and 4′,6‐diamidino‐2‐phenylindole (DAPI, 1:5000; Invitrogen, D1306) diluted in BSA (3% in PBS) for 30 min at RT. .. Cells were treated with 0.02 U recombinant NA from Arthrobacter ureafaciens (Merck, 10269611001) in 100 μL PBS at pH 5.5 or, as a control, were kept in PBS with neutral pH.

    Article Title: Influenza Virus Infection of an Immunocompetent Organotypic Model of the Human Respiratory Mucosa
    Article Snippet: .. After three additional PBS washes, a 30min incubation with 2μg/mL streptavidin AF488 (Molecular Probes) diluted in blocking buffer was performed for samples stained with biotin-conjugated antibodies. .. Samples were washed again three times with PBS and mounted using Fluoromount-GTM mounting medium (Thermofisher).

    Article Title: Generation of an Induced Pluripotent Stem Cell-Derived Alveolar Type II In Vitro Model to Study Influenza A Virus Infection and Drug Treatments.
    Article Snippet: In order to stain α2,6- or α2,3-linked SAs, cells were incubated with SNA-biotin (20 μgmL−1; Linaris, B-1305) orMAH-biotin (20 μgmL−1; Linaris, B-1265) in BSA (3% in PBS) for 1 h at RT. .. Cells were washed thrice in PBS and stained with streptavidin-AF488 (3.3 μg mL−1 for SNA or 10 μg mL−1 for MAH; Invitrogen, S-11223), and 4′,6-diamidino2-phenylindole (DAPI, 1:5000; Invitrogen, D1306) diluted in BSA (3% in PBS) for 30 min at RT. ..

    Labeling:

    Article Title: Protective and pathogenic antibody responses from a primate Shigella outbreak inform vaccine design
    Article Snippet: .. Cells were then stained with the antigen of interest (OMVs labeled with Vybrant TM DiO or DiD solutions [Thermo Fisher], IpaD labeled with biotin [Avidity BirA500] and coupled to streptavidin-AF488 and -AF647 [Thermo Fisher], or IpaB labeled with NHS esters of AF488 and AF647 [Thermo Fisher]). .. Cells were then stained with the antigen of interest (OMVs labeled with Vybrant TM DiO or DiD solutions [Thermo Fisher], IpaD labeled with biotin [Avidity BirA500] and coupled to streptavidin-AF488 and -AF647 [Thermo Fisher], or IpaB labeled with NHS esters of AF488 and AF647 [Thermo Fisher]).

    Incubation:

    Article Title: An unsuspected physiological role for mGluRIII glutamate receptors in hippocampal area CA1.
    Article Snippet: Then, we prepared 100 μm-thick sagittal sections of PFA-fixed brains using a vibrating blade microtome (VT1000S; Leica Microsystems, Buffalo Grove, IL). .. The slices were incubated with streptavidin AF488 (1:750) and Triton X-100 0.2% overnight at 4◦C, washed 3 times with PBS for 15 min, and mounted on glass slides using Fluoromount G DAPI mounting medium (Cat# 00-4959-52, ThermoFisher Scientific, Waltham, MA). .. All histological images were acquired on a Nikon Ti2E inverted confocal microscope equipped with 405/488/561/640 nm laser lines and Nikon Elements image acquisition software version 6.10.01.

    Article Title: Genetically diverse influenza antibodies highlight the role of IG germline gene variation and inform population-comprehensive vaccine strategies.
    Article Snippet: Fluorochrome-conjugated anti-human mAbs detecting cell surface CD19, CD20, IgM, IgD and IgG (BD Biosciences, BioLegend) were used to stain PBMCs. .. Biotinylated H1pdm2009-like influenza A monomeric head, trimeric stem domain and full trimer probes 39 were conjugated to ExtrAvidin®-PE (Sigma-Aldrich), Streptavidin-APC (Thermo Fisher) or Streptavidin-AF488 (Thermo Fisher) fluorophores and incubated with the stained cells. .. HA-antigen specific single cell sorting was performed using a FACSAria Fusion cell sorter (BD Biosciences) and target B memory cell populations were gated as live CD19+CD20+IgM–IgD–IgG+HA-trimer/head/stem+.

    Article Title: Genetically diverse influenza antibodies highlight the role of IG germline gene variation and inform population-comprehensive vaccine strategies
    Article Snippet: Fluorochrome-conjugated anti-human mAbs detecting cell surface CD19, CD20, IgM, IgD and IgG (BD Biosciences, BioLegend) were used to stain PBMCs. .. Biotinylated H1 pdm2009 -like influenza A monomeric head, trimeric stem domain and full trimer probes were conjugated to ExtrAvidin ® -PE (Sigma-Aldrich), Streptavidin-APC (Thermo Fisher) or Streptavidin-AF488 (Thermo Fisher) fluorophores and incubated with the stained cells. .. HA-antigen specific single cell sorting was performed using a FACSAria Fusion cell sorter (BD Biosciences) and target B memory cell populations were gated as live CD19 + CD20 + IgM − IgD − IgG + HA-trimer/head/stem + .

    Article Title: Influenza Virus Infection of an Immunocompetent Organotypic Model of the Human Respiratory Mucosa
    Article Snippet: .. After three additional PBS washes, a 30min incubation with 2μg/mL streptavidin AF488 (Molecular Probes) diluted in blocking buffer was performed for samples stained with biotin-conjugated antibodies. .. Samples were washed again three times with PBS and mounted using Fluoromount-GTM mounting medium (Thermofisher).

    Blocking Assay:

    Article Title: Influenza Virus Infection of an Immunocompetent Organotypic Model of the Human Respiratory Mucosa
    Article Snippet: .. After three additional PBS washes, a 30min incubation with 2μg/mL streptavidin AF488 (Molecular Probes) diluted in blocking buffer was performed for samples stained with biotin-conjugated antibodies. .. Samples were washed again three times with PBS and mounted using Fluoromount-GTM mounting medium (Thermofisher).



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